王亦生博士
姓名 王亦生博士
現任 研究員
所屬單位 中心副主任


Ph.D., Chemistry, National Taiwan University, 2001
名片
研究方向/領域

研究方向/領域

基質輔助雷射脫附/游離法 (MALDI) 是蛋白質質譜分析上最重要的離子源，但是其脫附/游離機制至今仍未被完全理解，其中的問題包含了複雜的生物分子解離動力學、基質與生物分子的共晶過程等 等。本實驗室將運用交叉分子束的概念，研究MALDI方法的解離機制，並更進一步研究氣態生物分子的光解離性質，期以此研究突破生物質譜技術的偵測瓶頸。 此外，本實驗室也進行新生物質譜技術研發，未來也將致力於開發質譜儀與其他研究設備的連結介面，期能幫助鑑定疾病並監測治療反應。
 

Dual-polarity imaging_mass
Dual-polarity imaging mass spectrometer and analytical results of insulin A-chain with matrix on surface. 
經歷
  • 2023-present Deputy Director, Genomics Research Center, Academia Sinica
  • 2019-present Professor, Genomics Research Center, Academia Sinica
  • 2011-2019 Associate Professor, Genomics Research Center, Academia Sinica
  • 2005-2011 Assistant Professor, Genomics Research Center, Academia Sinica
  • 2002-2003 Postdoctoral Fellow, National High Magnetic Field Laboratory (NHMFL), Florida State University, U.S.A.
  • 2001-2005 Postdoctoral Fellow, Institute of Atomic and Molecular Sciences (IAMS), Academia Sinica
榮譽
  • 2004 Outstanding Post-Doctor Researchers Conference Travel Grant
  • 2002-2003 Advanced Abroad Research Fellowship
  • 2002 Outstanding Post-Doctor Researchers Conference Travel Grant
  • 2000 Outstanding Students Conference Travel Grant
  • Yi-Hong Cai, Cheng-Huang Lin, Yi-Sheng Wang, 2023, “Theoretical study of general principle of high-resolution MALDI-linear time-of-flight mass spectrometry in middle to high m/z ranges”, INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 489, 117052, 117052. (SCIE)
  • Lai Yin‐Hung*, Wang Yi‐Sheng*, 2023, “Advances in high‐resolution mass spectrometry techniques for analysis of high mass‐to‐charge ions”, Mass Spectrometry Reviews, 42(6):2426-2445. (SCIE)
  • Chang Ko-Keng, Cai Yi-Hong, Hsiao Chih-Hao, Hsu Cheng-Chih, Wang Yi-Sheng*, 2022, “High-performance miniature linear time-of-flight mass spectrometry as an advantageous tool in a high mass-to-charge range”, The Analyst, 147(18), 4116-4123. (SCIE)
  • C.-H. Hsiao, Y.-H. Lai, S.-Y. Kuo, Y.-H. Cai, C.-H. Lin, Y.-S. Wang*, 2020, “A Dynamic Data Correction Method for Enhancing Resolving Power of Integrated Spectra in Spectroscopic Analysis”, ANALYTICAL CHEMISTRY, 92(19), 12763-12768. (SCIE)
  • Wang Chia-Chen, Wang Yi-Sheng*, 2019, “Effective analysis of degree of polymerization of polysialic acids in mass spectrometry by combining novel sample preparation and dynamic instrument optimization methods”, Carbohydrate Research, 471, 78-84. (SCIE)
  • Ou Yu-Meng, Kuo Shu-Yun, Lee Hsun, Chang Huan-Tsung, Wang Yi-Sheng*, 2018, “An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry”, Jove-Journal of Visualized Experiments, 137, e57660. (SCIE)
  • Cai Yi-Hong, Wang Yi-Sheng*, 2018, “Impact of uneven sample morphology on mass resolving power in linear MALDI-TOF mass spectrometry: A comprehensive theoretical investigation”, Journal of Mass Spectrometry, 53(4), 361-368. (SCIE)
  • H. Lee, Y.-H. Lai, Y.-M. Ou, C.-W. Tsao, Y.-J. Jheng, S.-Y. Kuo, H.-T. Chang, Y.-S. Wang*, 2017, “Enhancing carbohydrate ion yield by controlling crystalline structures in matrix-assisted laser desorption/ionization mass spectrometry”, ANALYTICA CHIMICA ACTA, 994, 49-55. (SCIE)
  • C.-C. Wang, Y.-H. Lai, Y.-M. Ou, H.-T. Chang, Y.-S. Wang*, 2016, “Critical Factors Determining Quantification Capability of MALDI-TOF Mass Spectrometry”, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, 374(2079), 20150371. (SCIE)
  • Y.-M. Ou, C.-W. Tsao, Y.-H. Lai, H. Lee, H.-T. Chang, Y.-S. Wang*, 2016, “Preparation of homogeneous MALDI samples for quantitative applications”, JOVE-Journal of Visualized experiments, 116, e54409. (SCIE)
  • Urban, P.; Chen, Y.-C.; Wang, Y.-S., 2016, Time-Resolved Mass Spectrometry, 320 pages, London: John Wiley & Sons, Ltd.
  • Chang, K.-K.; Cai, Y.-H.; Chen, C.-H.; Wang, Y.-S.;*, 2016, “Neutralization charge detection method for detecting ions under ambient and liquid phases.”, CHEMICAL COMMUNICATIONS, 52(29), 5187-5189. (SCIE)
  • Y.-H. Lai, Y.-H. Cai, H. Lee, Y.-M. Ou, C.-H. Hsiao, C.-W. Tsao, H.-T. Chang, Y.-S. Wang*, 2016, “Reducing spatial heterogeneity of MALDI samples with Marangoni flows during sample preparation”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 27(8), 1314-1321. (SCIE)
  • Cai, Y.-H.; Lai, Y.-H.; Wang, Y.-S.*, 2015, “Coupled space- and velocity-focusing in time-of-flight mass spectrometry – a comprehensive theoretical investigation.”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 26(10), 1722-1731. (SCIE)
  • Lai, Y.-H.; Chen, B.-G.; Lee, Y. T.; Wang, Y.-S.;* Lin, S. H.*, 2014, “Contribution of thermal energy to initial ion production of matrix-assisted laser desorption/ionization observed with 2,4,6-trihydroxyacetophenone.”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1716-1722. (SCIE)
  • C.-L. Wu, C.-C. Wang, Y.-H. Lai, H. Lee, J.-D. Lin, Y. T. Lee, Y.-S. Wang*, 2013, “Selective enhancement of carbohydrate ion abundances by diamond nanoparticles for mass spectrometric analysis.”, ANALYTICAL CHEMISTRY, 85, 3836-3841. (SCIE)
  • Y.-H. Lai, C.-C. Wang, B.-H. Liu, C. W. Chen, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2012, “Analysis of initial reactions of MALDI based on chemical properties of matrices and excitation condition.”, JOURNAL OF PHYSICAL CHEMISTRY B, 116(32), 9635-9643. (SCIE)
  • C.-H. Hsiao, C.-W. Hong, B.-H. Liu, C. W. Chen, C.-C. Wu, Y.-S. Wang, 2011, “Comprehensive Molecular Imaging of Photolabile Surface Samples with Synchronized Dual-Polarity Time-of-Flight Mass Spectrometry”, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(7), 834-842. (SCIE)
  • Y.-H. Lai, C.-C. Wang, S.-H. Lin, Y. T. Lee, Y.-S. Wang*, 2010, “Solid-Phase Thermodynamic Interpretation of Ion Desorption in Matrix-Assisted Laser Desorption/Ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114 (43), 13847–13852. (SCIE)
  • B.-H. Liu, O. P. Charkin, N. Klemenko, C. W. Chen, Y.-S. Wang*, 2010, “Initial ionization reaction in matrix-assisted laser/desorption ionization”, JOURNAL OF PHYSICAL CHEMISTRY B, 114, 10853-10859. (SCIE)
  • Liang, C.-W.; Lee, Y. T.; Chen, C.-H.; Wang, Y.-S., 2010, “Ionizing nonvolatile samples using laser desorption-proton-transfer reaction with cluster reagent ions”, International Journal of Mass Spectrometry, 291 (1-2), 61-66. (SCIE)
  • Chen, H.-K.; Chang, C.-K.; Wu, C.-C.; Huang, M.-C.; Wang, Y.-S., 2009, “Synchronized dual-polarity electrospray ionization mass spectrometry”, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20 (12), 2254-2257.. (SCIE)
  • Bo-Hong Liu, Yuan Tseh Lee, Yi-Sheng Wang, 2009, “Incoherent production reactions of positive and negative ions in matrix-assisted laser desorption/ionization”, Journal of the American Society for Mass Spectrometry, 20(6), 1078-1086.. (SCIE)
  • M.-H. Li, S.-T. Tsai, C.-H. Chen, C. W. Chen, Y. T. Lee and Y.-S. Wang, 2007, “Bipolar Ion Detector Based on Sequential Conversion Reactions”, Analytical Chemistry, 79 (4), 1277-1282.. (SCIE)
  • S.-T. Tsai, C.-W. Chen, L.-C. Huang, M.-C. Huang, C.-H. Chen and Y.-S. Wang, 2006, “Simultaneous mass analysis of positive and negative ions using a dual-polarity time-of-flight mass spectrometer”, Analytical Chemistry, 78 (22), 7729-7734.. (SCIE)